Chapter 295 Penicillin Experiment Penicillium Was Stolen!
Chapter 295 Penicillin Experiment—Penicillium was stolen!
The miraculous plant oxytocin - no, microbial oxytocin - gave Grete the confidence to launch the penicillin project. And this magical technique really lived up to his expectations. After a week, enough Penicillium was reproduced to be able to be used for experiments.
And the priest apprentices of the Natural God Church have bred enough rats for them to conduct a luxurious experiment...
In the dissection room on the first floor of the Master Tower, 24 apprentices were divided into six experimental tables, each facing a cage of rats. In the crowd, the two teenagers from the Natural God Sect looked nervous and whispered:
"Use it sparingly...Use it sparingly...Don't kill yourself by being careless..."
It's so pitiful that their experience of raising rats makes everyone cry when they hear it, and sad when they see it. Specialized breeding companies, no, specially formulated mouse feed, no, mice with stable conditions, no germs, and having lived in the laboratory for several generations, no...
It is up to the priests of the God of Nature to dig out the mouse holes themselves, catch them, and let the mother mice give birth to pups, and then raise them after they have given birth. If the mouse is irritable and frightened, use Animal Friendly to appease it; if it doesn’t know the feed ratio, its animal communication skills are top notch; if it wants to be free of germs, use healing spells every day. The priest's apprentice's healing skills were limited, and he was reluctant to use them even if he bumped into them. He just hit the rats.
It is not an exaggeration to say that rats raised in this way are the lifeblood of priests. Now take it out for experiments and use 120 of them at one go. It’s incredible!
However, raising rats for a hundred days and using rats for a while, Grete ordered to use them, but they couldn't help but resist. The four priest apprentices could only gather around the table and look at Gretel with sad eyes as he demonstrated to the students:
Lift the rat's tail with your right hand and place it on the lid of the rat cage;
When the mouse struggles to crawl forward, use your left thumb and index finger to grasp the mouse's ears and neck skin;
With the mouse in the palm of your left hand, use the ring finger and little finger of your left hand to pinch its back skin and tail...
"Do you understand everything? The movements must be steady and accurate. If you don't catch it well once, you have to let go and catch it again. Be careful of being bitten! - Come on, everyone can catch one by themselves!"
This sentence is like pouring a ladle of cold water into a boiling pan. In an instant, the entire anatomy room was noisy. Of the 24 students, 20 were shouting:
"Ouch! It bit me!"
"Run away! Run away!"
"Mr. Trocca! Help!—No, don't kill it!"
"Ahhh! I squeezed the shit out of it!"
The four priest apprentices of the God of Nature looked at each other in confusion. Can catching mice be a problem? Isn’t it just a matter of reaching out and grabbing it?
Gretel was already prepared for this. Well, when they first started doing animal experiments, the classmates in their class also looked like this, and they were no better than these spell casters. With great patience, he looked at each table and gave instructions one by one:
"Grab the tail quickly! Be accurate!"
"No, don't scratch the base of the tail! Grab the middle part of the tail!"
"You don't have to use so much force... Do you think you're going to have to break off your tail?"
"If it doesn't work, just carry it around a few times to make it faint... Hey, why did you throw it away on your own! Mr. Trocca, please help to catch it!"
"Then pinch its ears...don't pinch the neck! Don't press! Don't pull!!!"
With a click, the mouse died...
Grete rubbed his forehead. Pulling the rat's tail and pressing its cervical vertebrae at the same time is the way to kill a rat, classmate! I haven't learned this lesson yet!
"Okay, you can catch another one...Fortunately, I have a spare supply..."
Just the act of catching a mouse has gone through all kinds of upsets (Mr. Trocca was very popular among them). Finally, after much stumbling, the experiment reached the stage of subcutaneously inoculating the mice with Staphylococcus aureus.
Gretel showed them again. It is obviously a simple subcutaneous injection action - much simpler than intraperitoneal injection, intracranial injection, etc. - I have to go around the table again to correct all the wrong behaviors of the apprentices:
"Keep your hands steady! The more the mouse struggles, the more steady your hands must be! - Stop!... It's too late, you'll poke yourself..."
"Don't squeeze it so hard! The mouse can't escape! ...Forget it, it's dead after squeezing it, just change it!"
"Insert the needle flat! Don't be vertical! Don't be crooked! - Look, you pricked your finger!"
"Don't insert the needle too deep! Wait -" The blood is so fierce! Did you puncture a major artery or something?
"Subcutaneous! Subcutaneous! Insert the needle flatly, and then lift the needle up! Lift it flatly, not the needle tip upwards! Oops, the skin has been poked through... Forget it, insert the needle at another site!"
This kind of thing can be made by a skilled master with ease and ease, but by a novice who doesn't know how to make it, it can be made in a variety of ways and in a hurry. Even though Gretel was fully mentally prepared, they still made him feel confused and his blood pressure soared to 20 mm Hg.
It was not easy to complete the hypodermic injection experiment, during which 10 mice were killed, four students pricked their fingers, overturned penicillin test tubes, broke syringes, and countless other accidents. Finally, 80 mice survived and entered the observation stage...
"How's the mouse doing today?"
"normal."
"normal."
"Mine is normal too."
The students were calm. The mouse's condition was still normal on the second day, and on the third day...
"It doesn't eat much anymore!"
"I don't like to move anymore!"
"You look so lethargic!"
On the fourth and fifth days, the mice's condition deteriorated. By the sixth day, at least half of the group reported that the mice were getting worse, with facial bluish discoloration gradually becoming darker and darker.
"It's belly is also blue! Where the needle went in, it's all blue!"
"Sir, is it dying?"
Four priest apprentices from the Order of the God of Nature and two apprentices from the Temple of the God of War looked at Gretel eagerly, just waiting for his words. Gretel took a deep breath:
"Not necessarily. - Bring us the Penicillium mold we prepared!"
They divided the sick mice into four groups. One group was given oral administration of Penicillium, one group was injected with Penicillium subcutaneously, and one group was treated with magic... The other group did nothing and was left as a control. The apprentices wish they could live in the laboratory, staring at the mice with their eyes wide open...
"Oral Penicillium group. This one won't work..."
"Oral group. This one seems to be no longer good." A mage picked up the mouse and observed it. The priest of the god of nature turned his face and glared at him. The mage shrugged:
"What's wrong? I am a necromancer. Although we are not compatible with animals, I can feel the breath of death very strongly!"
"Hypodermic group.—Hey, sir, this one seems to be doing better!"
There was a crash, and 25 of the 24 students in the anatomy room suddenly gathered around. ——The one with more people is Master Lin En. He stretched out his two skinny arms on the outside of the crowd and tried his best to squeeze in:
"Yes, yes, it is indeed better! The aura of death is not so strong!"
One day later, 10 of the 20 mice in the subcutaneous injection group were alive and kicking and escaped death. This therapeutic effect is second only to the 20/20 of the magic group, and much higher than the oral group and the control group that all died.
"Congratulations, ladies and gentlemen." Gretel clapped his hands and attracted the attention of the students:
"You have successfully found a natural product that can effectively treat patients in places where magic cannot reach. Now, let us continue our research to find out the appropriate dosage of this product and make it into a potion , how to carry it long distance!”
"yeah!"
"Long live!"
"We made it!"
Twenty or so surgical caps flew up to the ceiling. White, gray, blue——
As for the material of the surgical cap, Gretel allowed them to use it freely, as long as it could wrap the hair tightly and not hinder the operation. So, in this wave of celebrations, he also saw two touches of green, one falling together, the other falling...
After a short celebration, the students entered the long experimental process. This time, more culture media were produced to promote the growth of Penicillium - sugar agar culture medium, blood agar culture medium, beef extract culture medium, wort culture medium, corn meal culture medium, potato sucrose culture medium...
The entire laboratory, no, the entire mage tower, seemed to have turned into a big kitchen. Steaming, boiling, boiling, crushing, filtering...it's steaming all day long. If the students don't want to eat, they can just drink the culture medium to fill their stomachs.
Of course, no one actually does this. In the words of Leon Carlos:
"I know that wort smells bad, but I really didn't know that beef extract smells worse than it...it's disgusting, this smell..."
Grete allows students to use their imaginations and experiment with various recipes. It is definitely a waste, but fortunately there is a fire element pool in the mage tower, which provides endless heat; those sea stone flowers, beef, sheep blood, you can buy a lot of them with just one contribution point, so you don't have to worry about not being able to afford them. Corn, potatoes, etc.?
Just kidding, that stuff doesn’t even count on money!
In the midst of the mass production boom, the four priest apprentices of the God of Nature have become leaders of the students. Especially the girl who caught the wild boar. She basically no longer does culture media. She spends two hours every day wandering in the laboratory, half-closing her eyes, sensing the culture dishes one by one:
"This...can't. It's basically not long."
"This...it grows a little slowly, you can keep an eye on it."
"This one grows up pretty fast."
"This...can you raise the temperature a little?"
I have to say that you can always trust the biological affinity ability of the God of Nature Priest. Of course, except Gretel, the impostor. After ten days, they had sifted out all meat and blood raw materials and began to specialize in corn flour, corn liquor, corn starch, wheat flour, potato flour...
After reaching this point, more than half of the 24 students were so tired that their eyes were crooked, their mouths were slanted, and their facial features were distorted. Grete himself felt a little unable to bear it, so he waved his hand generously and allowed them to go home and relax:
"Take a good rest for two days. When we come back, we will start a new project!"
The students let out a cheer and dispersed. Gretel couldn't wait to rush out of the mage tower and go to the beach to breathe fresh air. Just kidding, he has been doing experiments for so many days and he hasn’t even been exposed to the sun. If he doesn’t go out for a walk, he will be deficient in vitamin D!
Gretel played happily for most of the day. When I returned to the mage tower, I received a blow in the head:
Penicillin strains were stolen.
****
Today I asked in the group what accidents have occurred when you performed subcutaneous injection experiments on mice, and then I heard all kinds of blood and tears...
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