Chapter 294 Penicillin: Grow the Penicillium First!
Chapter 294 Penicillin...Grow out the Penicillium first!
Grete was extremely happy. He rushed out of the anatomy room on the ground floor and ran to the P4 laboratory upstairs. He began to cast blessing spells on himself one by one:
Oil the soles of your feet!
The lightness of cats!
Jump!
"That's enough for you!" Behind him, Master Lin En was holding his robe and ran up one floor before he was thrown half a floor away. Seeing Gretel, he couldn't help shouting:
"There are only two floors in total! You have cast three spells!!!"
With a meow, the black cat jumped up, expanded, expanded, and expanded again in mid-air, turning into a giant black panther with a shoulder height of 1.8 meters. He lowered his head, picked up Master Lin En's collar, and rushed up the stairs.
Gretel really couldn't be unhappy. From the time when he arranged this research project, from the time when he was still at the original site of the laboratory, searching for melons from all over the world, or even earlier, from the time when he rescued the old dwarf in the forging area and cultured tubercle bacilli from the old dwarf's sputum. , he was waiting for this day——
penicillin!
Or, other antibiotics that can secrete lysozyme! Streptomycin, oxytetracycline, even a little gentamicin would be good!
The only things that could stop him were the caution of a doctor and the fear of a massive leak of infectious bacteria as a researcher!
Even though Grete's hands were shaking with excitement, he followed the safety operating procedures and meticulously wore a mask, gloves, washed hands, and put on protective clothing. After all the students had finished dressing - by the way, Mr. Trocca was grabbed by the back of the neck by the golden skeleton as usual and rushed outside - he opened the door, entered, closed the door, and opened the inner air-tight door, layer by layer. Enter the core laboratory.
The P4 laboratory in the Mage Tower has been greatly modified compared to Gretel's original version. A particularly important point is that the original single channel has been made into three areas and two channels, animal culture area, anatomy area, bacterial culture area, and experimental area. Each room is independent, with clean channels in and contaminated channels out.
Do you want to enter the red zone and then retreat to the clean corridor like the last time the judges came to visit?
Impossible, the door has one-way access and is controlled by the tower spirit!
The large team of people rushed straight to the bacterial culture area. Under the guidance of the reporting student, Grete held up the petri dish and looked at the light carefully. Without the need for additional sampling, slide preparation, staining, or microscope, he could see the abnormality on the petri dish with his naked eyes:
Among the yellowish bacterial colonies that covered the bottom of the petri dish, there was a large area that looked translucent and looked like glass. On the outside of the blank area, large swaths of Staphylococcus aureus were squeezing together, but they could not make a step forward; in the center of the blank area, a small piece of green mold stood proudly and proudly!
that's it!
This is what I want!
Gretel turned around suddenly. Even though he was wrapped in a protective suit and his voice was buzzing, he couldn't stop him from raising his voice excitedly:
"Which group did this experiment?"
"Our group!"
A clear female voice answered. Gretel turned his head and saw a tall student, only half a head shorter than himself, stepping out from the crowd and standing in front of him. Considering that there are only two women in this batch of apprentices, and the other one is a little man of 1.5 meters, no need to ask, it must be the one who brought the wild boar.
"Very good." Grete nodded heavily, trying hard to suppress his intense heartbeat:
"As a group, your previous work will be suspended and you will concentrate on studying these green things in the petri dish.
——I want you to cultivate and purify it, find out why it makes bacteria afraid to get close, and prepare a large amount of that substance!
Congratulations, everyone, you may have found a new way to combat the plague! "
In the room, all 24 students were shocked. Especially the eight people in Black Crow Swamp, their breathing suddenly became heavy and they took a step forward reflexively.
It's a pity that even if their eyes are green, not everyone can participate in the project...
The third batch of students who passed the assessment were defined by Grete as poor students and were not qualified to participate in the project at all. They can only work hard and practice more, hoping that next time there is a new exam, they can turn around in one fell swoop. This alone eliminated 1/3 of the people.
The first and second batches are not accessible to everyone. Among the first ten people who passed the examination, the five who were infected with Mycobacterium tuberculosis should do whatever they were supposed to do——
Mycobacterium tuberculosis is not sensitive to penicillin but is sensitive to streptomycin. These five people, as well as the second batch of three subsequent people who passed the examination and joined their work, have to continue to work hard. Want to do something new for a change?
Thinking too much!
In the end, only the young lady from the Natural God Church, with two necromancers, a medical magician, a war priest, and three people who joined later, made penicillin in a dark way. According to the instructions of the young lady, each person is in a group and first makes various culture media and cultivates them in large quantities——
Then, Grete listened to them wailing in various ways for two days in a row.
"I'm dying..."
"Kurona, I'm done with this too... give me some more..."
"No, it's not possible. It's half-dead... Corona, why are you raising it so well..."
"Can't we necromancers keep this kind of thing? There is too much death aura on it! It can't survive with anything! Let me tell you, when I'm at home, I can't even keep catnip alive!"
"Don't talk nonsense, aren't you still feeding the bacteria?"
"Bacteria are plagues! Isn't it right for necromancers to feed plagues? But this thing is not a plague! It is to restrain the plague! It's normal if I can't feed it. If I can feed it, I will be killed by it. Got it!"
Grete snickered beside him. How can it be so easy to cultivate Penicillium? If the nutrient ratio of the culture medium is wrong, the pH is wrong, and the oxygen content of the air is wrong, it will either not grow or grow less, or it will not ferment or ferment less. Without fermentation, it will produce less penicillin, and it will be a waste of time...
In the history of the previous life, Alexander Fleming discovered penicillin in 1928, and after five years of hard work, he turned to sulfa;
In 1938, German chemist Ernst Chain began purification experiments, and by the winter of 1940, a little bit of penicillin was refined;
In 1941, Australian pathologist Florey increased the production of penicillin from 2 units per cubic centimeter to 40 units;
It was not until 1942 that mass production of penicillin began. So in those spy movies, people started grabbing penicillin during the Long March. Well, I suggest the crew turn their target to sulfonamide...
So many of the world's top scientists worked hard to create penicillin that could be mass-produced. If Gretel hadn't had the support of magical powers to work out miracles, he wouldn't have dared to take part in this project.
All in all, come on, boys and girls!
****
I’m so tired, I just wrote 4,000 words today…
There is one more update left, I will pay it back... I will definitely pay it back...
And worship, Fleming, the man who discovered penicillin, is such a tough guy! He worked at the age of 16, and received an inheritance of 250 pounds at the age of 20. His brother suggested him to study medicine, and then, in July of that year! Note that this is the year! Passed the exam in 16 courses! Enter the University of London!
You asked me to take a year off from work to review after four years of work, and I couldn’t even pass six courses...