Chapter 1503 Speed up the Progress, the Customer Will Lose Patience! (Platinum Tower Leader Will Add 6 Updates)
One daughter is married twice, one servant is married to two masters.
Borrow chickens to lay eggs, borrow flowers to offer to Buddha.
What should you do if you are caught doing something bad?
Danger!
Gretel, danger! ! !
The alarm in Grete's heart rang loudly. Making something for senior sister is nothing, Cerela is not a stingy girl, and she will not be unhappy because he makes something for senior sister in any case;
However, he claimed to be giving her a gift and delivered it to her to win her favor. At this time, she caught him and found out that he was using it as a cover to inspire her senior sister's research work...
Gretel estimated that Serira would probably be angry with him, very, very angry. The kind that can’t be coaxed in three days!
At the critical moment, Gretel remained calm and his heart did not beat, exerting his composure to the extreme. Looking directly at Serila, his eyes did not shift, and he smiled slightly:
"This is just for you! Look, Serira, these are the flowers you like:
These roses were planted in front of your house when we were on Elf Island; these osmanthus flowers were shaken down by you, and you even soiled a silk scarf for me to wash for you; these daisies were obtained by you from the magic plant. of……"
He checked everything and found out all the treasures. The smile on Cerira's lips grew bigger and bigger, and finally she smiled and grabbed Gretel:
"You are great! The taste of this snack is a bit bland, but it looks so beautiful! Grete, please teach me how to make it! I want to make something that tastes good and looks beautiful, so that I can scare my parents when I turn around!"
"Okay... wait a minute, let me say a few words to Senior Sister first... Senior Sister, when this thing is used to make chromosomes escape, it is best to purify it. Purity is very important...
You can add some conductive liquid to make it conduct electricity...
Pay attention to the time and voltage intensity of overcharging. You may want to try this slowly."
After saying a few words quickly, he was dragged out by Cerira, not even having time to put away the molds on the table.
Instead, Cerira rushed to the door, waved her hand back, and a whirlwind swept up all the tools on the table and followed her out...
"Little guy, you're pretty good at coaxing girls." Archmage Philby looked at Grete's back, shook her head and chuckled. After laughing for a while, he took a deep breath and raised his voice:
"Taling!"
"I'm here, Your Excellency the Archmage. What are your orders?"
"Arrange five invisible servants to come over and operate according to my requirements... Invisible servant No. 1 is responsible for weighing the agar powder... weighing 1 gram, 2 grams, 3 grams... until 10 grams each time;
Invisible Servant No. 2 is responsible for preparing distilled water and brushing test tubes. Should Nordmark Master have built in the requirements for brushing test tubes? "
"Yes, it's already built in. Your Excellency, Archmage, please continue to give orders."
"Okay. Prepare enough distilled water, brush 20 50 ml test tubes, 20 100 ml flasks, and 20 200 ml flasks. Invisible servant No. 3 is responsible for adding agar powder and distilled water to the test tubes. I will write down the ratio later. You, then shake the tube;
Invisible Servant No. 4 is responsible for adjusting the heating magic array..."
She rattled off a long list of instructions. In a moment, five invisible servants emerged from the magic circle reserved for invisible servants in the mage tower. They each performed their duties and began to work.
Archmage Philby sat in front of the experimental table, took a long breath, and then let out a long breath. Then, he focused on the parchment in front of the experimental table and began to recheck the experimental plan:
Regarding the concentration of agar powder, do you need to make several more gradients?
After the agar powder is dissolved and heated, to what degree should it be heated, or should it be boiled directly? If it is boiled directly, how long does it take to cook?
What should be added to agar gel to make it conduct electricity better?
How should the cut chromosome spots be stuffed into the frozen agar block to facilitate the passage of electricity?
Also, Little Gretel said that the agar needs to be purified - how to purify it? To what extent is it purified?
Which kind of purified product can allow the cut chromosome spots to expand in the agar gel?
As an 18th-level archmage, she has a large number of invisible servants, golems, and disciples to run errands for her with just one thought or order.
And what she has to do is to constantly think, verify, modify, verify, then overthrow her plan, and then verify again...
In the process of thinking, the first plate of agar gel has been made. Well, it turns out that agar cannot be completely dissolved unless it is boiled. It must be boiled in boiling water for more than 10 minutes.
In the heating experiment, the batch that is not boiled can be directly crossed out...
20% content, 19% content, all the way to 1% content, were neatly lined up in front of her, like a plate of snacks in a snack shop.
Archmage Philby raised her fingertips slightly, and a thin stream of electricity came out of her hand, injected from one end of the agar gel and moved to the other end.
Well, the concentration of agar gel seems to have nothing to do with conductivity. But, on another level, the more concentrated salt water you add to it, the more conductive it becomes.
With another raise of his fingertips, a small invisible piece of chromosome cut out from the magic circle was mixed with salt water and injected into the hole dug in the agar gel.
The second ray of current was injected, so delicate that you could see the mental power of electrons, and you could feel the tiny chromosome fragments, so subtle that they were completely invisible to the naked eye, being pushed forward by the current.
The agar gel that seemed crystal clear to the naked eye showed countless holes under the scanning of mental power. Large and small, like a large piece of sponge or a large piece of cut cheese.
And those chromosome fragments were moving forward, forward, in the holes of the sponge and cheese, trying to pass through the holes... and they got stuck...
Still too big?
It is not a whole bunch of chromosomes, nor is it a chromosome, but a point cut off from a chromosome strip. Is it still too big for these holes?
The chromosomes, which are like balls of wool and twisted into a ball, cannot pass through these holes, and even a small cut-off point cannot pass through the holes...
Archmage Philby frowned tightly. To break it up, break it into little pieces?
"Danika, Arsu, Portus!" She contacted the tower spirit, sent a voice to the 11th floor, and named her three disciples:
"Come up! I have a task for you!"
Danica is responsible for purifying agar, and she has various purification magic circles and various potions at her disposal, but Philby only wants results;
Arzu was tasked with finding a potion that would better allow electricity to pass through the agar gel;
Portus is responsible for tossing about chromosomes, magic, potions, electricity, magnetism, and using them in various ways. The request is also to break them into pieces...
The order given by the senior sister through the tower spirit can be clearly understood by Gretel after a little communication with the tower spirit. He couldn't help but smile slightly:
Senior sister, come on. Although I don’t know what the buffer is, I probably know that it is a very troublesome thing, and it cannot be prepared by simple high school chemistry;
I don’t even know what the formula of the fluorescent dye is;
Chromosomes need to be purified and broken. There seem to be many ways to purify them. Anyway, I don't know any of them - well, it seems that you can add some acid, some alkali, some glycerin or something?
Can enzymes be used? Which enzyme can be used?
In short, running electrophoresis is a very big project, and it is all left to you...
He clapped his hands happily, feeling that he had gotten rid of a very difficult and big topic, which was really very clever. Well, the next trip is to go to the Nature Mission!
In the forest outside the mage tower, a group of nature priests raised an earth wall, turned the earth into stone, and planted shrubs and vines outside the wall to create a comfortable little house.
In the laboratory next to the residence, Annevia stood next to Elder Erwin, staring at the two lizards through the glass cabinet. Ashe Yuege stood opposite them and explained to them carefully:
"During the mating process of lizards, it is very important to have sufficient energy supply, which can significantly affect the level of the little lizards. However, using a magic circle to provide them with energy can easily alarm them and prevent them from completing mating normally..."
A wisp of light green breath flew out from her fingertips and landed on the magic circle in the center of the cabinet. The two lizards quickly became active, crawled in the cabinet for a while, and then climbed onto the magic circle and stacked on top of each other.
After they started, Ashe Yuege reached out and pressed. The magic circle gradually lit up, and the condensed natural power grew from weak to strong, and penetrated into the two lizards.
"What are you studying?"
Grete opened the door and walked in. Several people were staring at the lizard in the cabinet, and no one looked back at him. It wasn't until the two lizards separated and the female lizard lay motionless on the magic circle that Elder Erwin turned back with a smile:
"Isn't that your topic? After the dragon eggs are laid, they should hatch as many times as possible. If there are no dragon eggs or baby dragons, we will try it with lizards first - no, we are letting them mate."
Mating, laying eggs, hatching. Group testing, checking chromosomes... there is a lot of work to be done...
"What about artificial insemination?"
"Another group is doing artificial insemination. They take out the female lizard's eggs, inject the male lizard's sperm, and then put them back after cultivating them for a period of time. Another group is doing it..."
Ashe Yuege made a lot of complaints when talking about this:
"Grete, how long will it take for these projects of yours to be completed? When will they be completed? I see that those dragons seem to be in a hurry..."
"Don't be hasty, don't be in a hurry. This topic of increasing dragon fertility and dragon egg hatching rate will not be pursued for ten years. In fact, on the topic of dragon egg hatching, I can finish reading the literature in one year. Thank God." ”
Gretel tried to appease them:
"The problem of enhancing the body of the young dragon is more urgent. Well, I remember that I measured various information about the young dragon, as well as the chromosome points, and arranged for the elders to conduct targeted simulations. How is the simulation going now?"
This subject must be completed quickly. The young dragon's parents have already come to ask twice. In another six months, they will probably lose their patience!
When running gel electrophoresis, agarose is used instead of agar... Agarose is extracted from agar. The extraction methods include disodium hydrogen phosphate-citrate buffer method, flocculant-resin combined method, etc....
Before running electrophoresis, DNA needs to be isolated and plasmid DNA prepared. Of course there are many methods, including ethidium bromide density gradient method, two-phase method, acid phenol method, alkali denaturation method, methylated albumin (MAK) column chromatography, nitrocellulose method, electrophoresis method, etc... Senior Sister I don’t know any of them, not only don’t I know them, I’ve never even heard of them…