Chapter 1531 Gretel, You Finally Look Like an Evocation Mage!
(ScarletC will be rewarded with more rewards)
One hundred microinjections a day.
Ten days, a thousand times in total.
Grete looked sad while doing the experiment. After completing the experiment and analyzing the reasons for the failure of the experiment, I became even more sad:
"The injection force was too strong, the angle was wrong, and the fertilized egg was penetrated... This data must be excluded..."
"Too much cytoplasm was extracted? Should we control the amount of extraction next time?"
"Or, the nucleus was accidentally extracted? The part sent into the fertilized egg contained chromosomes, and the result was triploidy?"
"Or maybe the fertilized egg has a hole poked in it, and it was too damaged to begin with? Do you want to give it a healing spell to let the cell membrane heal naturally?"
"Or, should we beat the unfertilized eggs into a paste, put the fertilized eggs inside, and let them absorb nutrients naturally?"
"Ah... it's so difficult... how can we make the fertilized egg feel that the things sent in are for it to ingest..."
Gretel held his head in his hands, knocked his head on the experimental table, and groaned. From behind, Cerela walked over lightly and reached out to rub his shoulders:
"Okay, if we can't figure it out, let's change our thinking. Or we can go a little slower. Don't be so anxious. Your eyes are almost closed... It doesn't matter if you don't make a contract. I always believe in you!"
Grete took a long breath, straightened up, and wanted to lean back, but stopped at the last moment before reaching Cerela. He leaned forward again and buried his forehead between his hands:
"It's not for this...Celera, it's not for this. My intuition is that this road should work, but I haven't found a way yet... There must be a way, there must be a way..."
In previous lives, we even removed genetic material from animal cells and stuffed it into egg cells to promote the development of egg cells. What else can't be done? It must be that he didn't find the right method...
But where is the method? He is not an expert in cloning, he is not even an expert in cell research, and he has never even been exposed to the profession of biomedicine!
"If you can't think of a way, just do it randomly." Regarding this, Cerira was very calm, even so calm that she didn't care:
"Didn't Gretel say that? The so-called research means to do it according to the idea if you have an idea, and to do it randomly if you don't have an idea~~~ Especially when you are working with materials, you have to use all the materials at hand, this and that, to Pour inside..."
"Stop, stop, stop, stop -" Grete interrupted with a headache and raised his hand:
"When did I ever say that?"
"It was when Mr. Norwood was studying that...that...that rubber!" Cerela said confidently:
"He had no idea at the time. That's what you said. There are so many materials in the Mage Tower, just pour them into the rubber!"
"But it's not possible to study fertilized eggs." Grete couldn't laugh or cry:
"A fertilized egg is such a delicate thing. If the temperature is a little higher, it will die. If the temperature is a little lower, it will die. If the culture medium is thicker, it will die. If the culture medium is a little lighter, it will die..."
It's like growing bacteria in a laboratory. Bacteria that obviously grow very vigorously in the outside world and can grow large colonies on a salted fish. In the laboratory, the temperature was 0.1 degrees higher today. Ah, I'm dead...
The experimenters responsible for cultivating bacteria were so overwhelmed that they shed tears on the spot.
"Just die!" At this time, Serela fully demonstrated the arrogance of the dragon clan as a superior race, or in other words, the willfulness of the eldest lady as the silver dragon princess. He raised his chin and waved his hand vigorously:
"Anyway, these devil frog eggs are going to die! Anyway, they hatch normally, but a large part of them will not hatch, and a large part of them will not grow to adulthood! Grete, you can do whatever you want!
Add acid, alkali, salt, heat and freeze, gravity, magnetic current... do it all again! I think there is always a way! "
"Well..." Grete held his chin and really thought about it seriously. I'm afraid adding acid, alkali and salt won't work. The fertilized eggs will be corroded and problems will occur in minutes;
You can try heating and freezing, but you can only try it slightly. You can't denature the protein, crystallize the water in the cells, or puncture the organelles;
Gravity, magnetism, electric current, ultraviolet rays, and various rays. In fact, you can try them all!
Done!
Grete jumped up and started experimenting with the attitude of breaking a jar. Put it into the water bath, increase the temperature by 0.1 degrees, then 0.1 degrees higher, then 0.1 degrees higher... and increase it little by little;
Then put it into the freezing magic array, and the temperature will drop by 0.1 degrees, then by 0.1 degrees, and then by 0.1 degrees again...
The gravity field is more troublesome, so I simply put it in the centrifuge, use centrifugal force instead of gravity, and spin around and around;
The coil is set up, the magnetic field is pulled up, the pulse current is turned on, and the ultraviolet rays are illuminated; next to it is a laboratory, where all kinds of radiation, alpha rays, beta rays, and gamma rays, are illuminated one by one!
After such a series of experiments, it was finally seen that the fertilized eggs of the devil frog had undergone various changes. The vast majority of cases result from hatching failure, hatching malformation, or fertilized eggs dying halfway;
There are also cases where the chromosomes inside are completely disintegrated when exposed to rays and can no longer divide, such as the result of strong ultraviolet rays;
However, the results of pulse current stimulation really gave Grete a little surprise:
"Cerilla, look!" Gretel was lying in front of the microscope, his eyes shining brightly:
"The fertilized eggs seem to be developing better!"
"Where? Where where?"
Cerira quickly pushed aside and lay down in front of the microscope, trying to observe. However, she was not like Grete who followed the whole process, nor was she like Grete, who carefully observed every fertilized egg with a microscope and accumulated sufficient experience.
She looked left, right, up and down, but couldn't see anything. She could only look back at Grete blankly:
"...Where did you see it?"
"It's just that it looks stronger." Gretel didn't know how to describe it to her for a moment, so she could only make a few gestures feebly:
"Anyway, you see, according to the previous records, it should have developed to the blastocyst stage in an hour. But now, it has reached the blastocyst stage, so fast! So, give it a little electrical stimulation, and it will Sure enough, it can grow better!”
"That's great!" Although Cerela couldn't even understand the difference between a fertilized egg and a blastocyst, her little face lit up when she saw Grete's joy:
"Then electrocute it! Try to electrocute it! Electrocute it a few more times, use various methods to electrocute it, and try to electrocute it more and more!"
Gretel was also greatly encouraged. Since giving electrical stimulation can make fertilized eggs develop better, we should give more electrical stimulation in different ways.
——The best is to be able to summarize an effective method, or even magic, so that magicians can provide services to female dragons who need to be pregnant.
If the female dragon can learn to look inside her own ovaries and fallopian tubes and operate them for herself, then of course it would be very good...
With such expectations, Gretel devoted himself to research with great vigor. Then, he encountered a series of failures:
"The fertilized egg is finished, and there is a big hole in the cell membrane... Is the current too strong?"
"What is the current intensity? Wait, I remember there is something called transmembrane voltage. I have to think about what it is... Theoretically, it should just exceed the transmembrane voltage, just enough to break the cell membrane. Get a piercing!”
"The fertilized egg is finished again... This time it feels like it's not a problem with the current, but a problem with the pulse frequency? Well, adjust the pulse frequency again..."
"It's over again... I know, fertilized eggs and unfertilized eggs must be close together, even if they are even slightly apart! If there is even a slight distance, ten times the current will be used to make up for it, and the fertilized eggs will be burned. !”
"Well, the cytoplasm flow is reversed...it actually flows from the fertilized egg into the unfertilized egg. This is not what I want! I am not doing cloning...I understand, the pulse current direction is reversed!"
"Why is something not right this time... Wait, the direction of the pulse current is crooked! Wrong! If the direction is crooked, it will cause unnecessary large holes... But how to determine its direction... Micro-ranging magic array How to set it up?"
"It still doesn't work. There are still too many egg cells dead, and there are holes at both ends... I know, the transmembrane voltage between the two poles of the cell membrane and the contact area exceeds the perforation threshold, and this doesn't work!
Only by making the small area of contact between the fertilized egg and the unfertilized egg exceed the perforation threshold can the damage be minimized! But what to do..."
Grete was tossing the electric current to death. After struggling for half a month, Cerira was no longer willing to approach him——
Even when he was walking in the mage tower, electric charges were unconsciously floating around him, crackling, and shooting out a spark of electricity when he touched it.
On the contrary, Archmage Philby greatly appreciated Gretel. This is right, as a magician of the Evocation Department, as a magician of Thunder Horn, everyone has such a day!
This was the first time in such a long time that she saw that her junior brother was dedicated to researching the direction of electric current!
Little Grete, you finally look like a mage from Thunderhorn!
"Little Grete, how are you doing?" Taking a chance when no one was around, Archmage Philby stopped Grete and asked with a smile. Grete scratched his hair in annoyance:
"Senior sister, come here and take a look...I can't break through the last level..."
Seeing that the junior brother finally focused on the study of thunder and lightning spells, Archmage Philbi was in a good mood and patted her chest boldly:
"Don't worry, leave it to Senior Sister. Whatever problems you encounter, Senior Sister will help you solve it!"
Then, Your Excellency, the Archmage, plunged into things like current, voltage, pulse frequency, electric field strength, waveform, etc...
"Don't worry, I will definitely be able to figure it out! Give me three more days!"
"Three more days!"
"Three more days!"
Gretel: ...Sister, come on! ! !
This chapter refers to "The Effect of Different Electrofusion Conditions on the Fusion and Early Development of Recombinant Embryos of Mouse Cumulus Cell Nuclear Transplantation", "Production of Transgenic Fish by Electric Pulse Method", "Simulation and Experimental Research on Improving Cell Electrofusion Efficiency with Nano-Microsecond Composite Pulses" ", "Electrical Pulse-Assisted Activation of Human Oocytes", "Research on High-Throughput Cell Electrical Fusion Method Based on Microelectrode Array" and other papers... I am exhausted...