Chapter 228 Gretel Plague Company Nordmark Arrives! (Mr. Chun, Leader of the Alliance, Adds 7 Updates)
Chapter 228 Gretel Plague Company Nordmark arrives! (Mr. Chun, leader of the alliance, adds 7 updates)
Faced with the bloody facts (some rabbits actually spit out pink bubbles), Gretel was finally sent down the mountain. The old man in overalls escorted him to the train station, following him step by step, reluctant to leave:
"You should hurry up! We have already made 30 high-precision microscopes!"
"Everything that has been magnified 100 times has been sold. No one will buy anything that has a higher magnification. I'm just waiting for your paper to come out to trick those guys from Black Crow Swamp!"
Gretel: "..." You have to speak conscientiously! What a pit! Please translate for me, what is the pit of **!
Given the size of the bacteria, it can only be seen under a microscope! Only by increasing the magnification to 1000 times can we identify the characteristics of bacteria! Only by developing bacterial staining, various testing methods, and animal experiments can we determine which bacteria caused which disease!
I provide them with research tools, pave the way for them, and make some money myself. Isn’t this the best of both worlds? He also said it was a trap. Where is the trap?
There was a carriage when he went there. After he came back, several carriages came to the laboratory one after another:
An alchemist specially sent him a custom-made shadowless lamp, installed it in a separate dissecting room, and debugged it for more than half an hour;
Another alchemist brought two space bags, one bottle after another, and put down all the dyes that could be found on the market and in the laboratory, as well as all the liquids and solids that can be dyed, as well as alcohol, sulfuric acid, and nitric acid. …a whole bunch of drugs;
The third alchemist who came to the door was ordered to settle with Gretel: the shadowless lamp had not been sold yet, and the microscope was settled with contribution points. In addition, Gretel received a total of 87 gold coins and 5 silver coins. 9 copper coins - a share of pencil sales.
It must be said that the production and sales capabilities of the Alchemist Guild are truly outstanding.
oops!
Rich again!
There are gold coins again!
You can make things again, no, you can make money to make mills and ghosts work, and gold coins to work!
Hearing the sound of jingling coins, Gretel was overjoyed.
He had just finished packing his things, and soon the fourth, fifth, and sixth vehicles arrived - this time it was not a carriage, but a human-pulled flatbed truck. There was a cart full of rabbits, a cart full of rabbit feed, a cart full of seaweed and seaweed, and a few large pieces of beef and mutton on top. The color was bright, and it looked like they had just been killed today.
Ah...how much does this cost...
Bernard's eyes sparkled next to him. Grete glanced at him, pursed her lips, and took out her wallet with heartache. My gold coins, my little money, have to say goodbye before they are warm...
"This...you don't need to pay for it, you don't need to pay for it." The boss who followed the escort hurried forward, nodded and bowed, and waved his hands repeatedly: "The council has already paid!"
Gretel turned to look at him. The boss was wearing a bronze velvet jacket, with the collar and hem turned white, and a small six-pointed star embroidered with blue silk thread on the hidden cuffs. Throwing an identification technique at it, a shadow appeared on the six-pointed star, which was obviously a supplier authorized by the parliament.
Gretel felt slightly relieved. Looking further down, the boss's high leather boots were splashed with mud and exuded a fishy smell. It was obvious that seaweed had been transported from the port area and shipped directly here. He ran from behind the second carriage to the front, nodded and bowed, and handed over a sheet that was folded in half:
"My lord, please check. If the quantity is OK, I will go and settle the bill with the council."
Grete glanced at it and saw a dark skull mark flashing on the lower right corner of the list...
He opened the list. A plume of black smoke rose from the mark, and the skull's jaw opened and closed, making a dry mechanical sound:
"Little Gretel, hurry up, the plague branch is waiting!"
Grete: "..." No, this project has been transferred to Black Crow Swamp?
The Alchemy Guild is so quick! Or do you not want to contribute money, just use your previous paper to trick Black Crow Swamp into becoming a sponsor and put out the project list?
Regardless, it's nice to have someone pay. Grete suddenly stopped being speechless, his heart stopped hurting, and his head straightened up. He glanced at the list, turned his head, and commanded the barbarian:
"Bernard, go count the rabbits! There are 20 rabbits in the car. Check whether the number is correct and whether each one is healthy!"
Experimental animals are in place, experimental equipment is in place, and various experimental materials - such as various dyes used to stain bacteria, sea stone flowers used to make agar, beef and mutton used as broth culture media... are also in place one by one. Grete retreated and poured out his penicillin happily.
Well, the environment in the laboratory is really good...no worries about food, no worries about clothes, no worries about wind and rain. Except for a little noise in the laboratory and the sound of horse hooves passing by outside from time to time, there was basically nothing to fault.
Of course, this kind of sound of horse hooves is also because the city of Nevis has expanded the warning area to include his laboratory, so he still feels very safe...
"Ah... do experiments, do experiments, do experiments..."
Doing bacterial culture experiments is actually very boring. In particular, you have to cook the culture medium yourself, dye the color yourself, use iodine and so on, and start by burning the seaweed yourself...
If you are just a science enthusiast and want to do an experiment for fun, then forget it. Making results adds a sense of accomplishment. It doesn't matter if you can't make results. You've already enjoyed the process anyway.
However, Gretel-san must rely on these simple experimental conditions to really produce results...
"Ah - there are still 500 samples to be dyed ahhhhhhhhhh~~"
In the empty laboratory, where he was the only one, Grete let out a heartfelt wail.
Bacterial staining is a technical job, and it is also a job that relies on materials. In Heartland City, Grete searched for dyes all over the city and tried everything, but he only found a dye extracted from wood, which could dye cells a light blue.
The reserves of the Magic Council are much richer than those of the small Heartland City. There are three to four hundred kinds of dyes sent to the laboratory - including the use of acids, alkalis, and various chemicals. This workload is becoming more and more terrifying to the point that people dare not look directly at it.
But with such a large amount of materials, apart from increasing Gretel's fatigue, there is not much result - except for a red dye, other dyes either cannot be dyed, or can be washed away with just a wash of water.
"I want gentian violet! Crystal violet!" Grete flipped the table angrily. There is iodine, there is red dye, and if you give him gentian violet, he can distinguish between Gram positive and Gram negative! However, the premise of everything is to let him get Gentian Purple!
Grete wrote angrily, pressing the sharpened pencil against the white paper and poking two holes in a row. His demand list was brought back by the supplier boss. On the third day, a magician made a special trip to his door and delivered a space bag of materials:
"This is our newly found purple dye. This is conch purple, this is mountain violet, this is lac bug powder, this is lithospermum violet... This is the solution extracted from gentian flower. Although it is not purple, we also I brought it here. By the way, there are two bundles of gentian flowers here...and there is no gentian here, but there are different kinds of snake galls and fish galls here. You can use them as you like..."
Gretel: "T_T..."
How did that Gram senior find gentian violet back then? What's the chemical formula of this thing? How is it synthesized?
Grete searched hard and vaguely remembered that this thing was a mixture of tetramethylpararosaniline chloride, pentamethylpararosaniline chloride, and hexamethylpararosaniline chloride...
The name alone is a mouthful enough. If it weren't for the memory that comes with traveling through time, he wouldn't even be able to recite the official name. As for how to make this thing, Grete said that he is not a chemical major... Hey, has the pharmacy major next door done gentian violet?
It's so hard for me, I have nothing...
The only way to relieve worries is dissection. Grete spawned and purified the bacteria he had created, spawned and purified them again, and used different methods to toss them on the rabbits:
Mix it into the feed and feed it to the rabbits;
Let the rabbits' noses and respiratory tracts be wiped;
Make a small incision on the skin of the rabbit and apply it on the skin wound...
Then, observe, measure physical signs, and record the course of the disease. When the rabbit is dead, or half-dead and dying, kill it again, cut open its organs one by one, and observe the damage to the organs.
Fortunately, he can still dissect, and he can still dissect dozens or twenty rabbits every day to maintain his touch... Every day he cultured, cultured and stained, he would have thought that he was no longer a surgeon and had switched to a laboratory...
"Hmm... Rabbit A03, the whole body is dehydrated, the skin is dry and inelastic, the subcutaneous tissue and muscles are dehydrated, the heart, liver, and spleen are shrunk due to dehydration... the small intestine is obviously edema... the kidneys are necrotic..."
Gretel took a look and made a note. After writing everything, I quickly glanced at the previous records:
"Feeding at 8:05 am on the 21st. The disease started at 2:37 pm... The excrement was like rice swill... The excrement was smeared and the results were as follows..."
In the corner of the record book, there is a hand-drawn schematic diagram. The bacteria on it are like commas and a group of tadpoles, arranged in groups and active.
Very good. Combining the shape of the bacteria, the onset process, the time of death and the autopsy results, it is basically certain that this thing is 90% likely to be Vibrio cholerae.
Gretel showed a satisfied smile. Following the numbers on the record book, he carefully took out two petri dishes and kept them separately. One is used to give account to the Necromancy School, and the other is kept to prepare the cholera vaccine——
Living in this world where you don’t need anything and rely entirely on physical fitness and treatment, there are not many vaccines around you, and you always feel that your sense of security is very low.
Looking around the locker again, Gretel's smile became even brighter. Suspected Staphylococcus aureus, culture completed; suspected Pseudomonas aeruginosa, culture completed; suspected Shigella dysenteriae, culture completed...
Including today's Vibrio cholerae, he has cultured, isolated, and verified seven kinds of pathogenic bacteria. With three more, he can complete the exam!
Ugh...but why haven't penicillin been found yet...
Obviously he has worked so hard. Every day he spreads a table of petri dishes and sprinkles soil, dust, and everything he can think of into the culture media of Staphylococcus aureus, Mycobacterium tuberculosis, and various bacteria...
Why? Lady luck, why doesn't she favor him?
"Ah ah ah - it's getting cold. If it drags on any longer, there will be no more melons!"
Penicillin’s lucky charm! The strongest culture medium! If the weather gets colder, there will be no more antibiotics. He wants to get this antibiotic quickly, but there will be even less hope!
(Another three thousand words! Including noon, another extra update!)
Human technological progress is really a relay climb.
Hans Christian Gram created the Gram staining method, which is indeed very, very great, but it also benefited from the fact that before him, I don’t know which chemist made the gentian violet dye...
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