Chapter 156 Gretel’s Animal Experiments (Second Update)
In the next half month, in addition to attending regular lectures, Grete plunged into animal experiments. He, Matthew, each has a beak protective suit. As for Bernard, he had no choice but to use bubble surgery + gauze mask to help with the heavy lifting——
By the way, the barbarian's constitution is +2? Or Fortitude +2? In short, Grete felt that the most that could cure his illness was to make Bernard sneeze...
All in all, the safety of experimenters is reassuring for the time being. Gretel designed the process as carefully as possible, and with the help of Pastor Matthew, began to conduct animal experiments:
All healthy animals are placed in one cage per cage. Pay attention to the placement and air flow direction, and try to prevent the air between cages from flowing to each other to avoid cross-infection of experimental animals (the friendship of Pastor Matthew prompted the creation of vine and grass weaving cages, calling on the ground The earth rises to form walls and partition cages);
One-third of the experimental animals were placed in each cage, and one bat was placed in each cage to observe whether the experimental animals would become infected if they lived with the bats;
One-third of the experimental animals were treated in different ways: adding bat urine, bat guano, bat saliva, and dust wiped off the bat body to the feed and drinking water respectively, or using cotton swabs to dip the secretions from the bat's mouth. Rubbing the nasal cavity of experimental animals;
The remaining one-third, housed in normal cages, served as the control group.
Feed every day, observe the animals every day, write records every day...
Grete was really grateful to Pastor Matthew. I can weave cages and build earthen walls. I can catch sheep, pigs, rabbits, and bats with perfect accuracy. I can never make mistakes when adding food and water. I can clean the animal cages without getting too dirty, and moving the feed without getting too hard. Without his help, this animal experiment would really not be possible for a day:
Grete himself tried to catch a rabbit once. He put his hand into the cage and fished for it for a minute, but he just couldn't catch it. In the end, he was kicked by a rabbit on the back of his hand. If he hadn't withdrawn his hand quickly, he might have broken his fingers.
Not to mention the stoats that are as fast as lightning, the goats that can gore people, and the black pigs that can weigh at least a hundred kilograms...
Gretel only glanced at it and gave up the idea of doing anything.
He stood aside and directed Pastor Matthew: "Grab this rabbit! Turn it upside down! Measure the temperature! Insert the thermometer! - Hey, don't put it in your mouth, I only have one thermometer, don't let it chew it! The mercury poisonous!"
"…Then where should I insert it?"
Grete stared into Pastor Matthew's eyes under the beak mask, tightened her lips, and resolutely refused to speak.
And it’s not because of the teacher’s instructions that Pastor Matthew works hard without complaint. In fact, every time he does something, he has countless questions to ask:
"Why do we need to prepare so many kinds of animals?"
"Because we don't know what kind of infection bats can infect." Grete looked at the cage with a look full of pity. So many kinds? I thought I was too little prepared!
In theory, mice, rabbits, dogs, pigeons, as well as pigs, horses and sheep known to be intermediate hosts must be prepared. If possible, it would be better to have civets and pangolins - well, forget about the latter, to protect animals...
"Why feed them the bats with... uh, different stuff?"
"Because we need to determine the transmission route..." Fecal-oral transmission, droplet transmission, and body fluid transmission, different routes require different defense methods. In terms of disease prevention, Grete has always shared endless knowledge:
"For example, if you get sick by breathing in its sneezes, you should cover your mouth and nose like we do... If you eat dirty things into your mouth, you must wash your hands before eating..."
"Then why do we need to prepare more than twenty of each type?"
"Because animal experiments are about betting on probability...oh, betting on possibility. There are so many animals, and if each method and each type of bat is given a point, there won't be much left."
Twenty is too much?
The little friend in the pharmacy field next door told me that whenever a new drug is tested, the first phase of clinical testing will measure half of the lethal dose, and no matter what, it will start with dozens or hundreds. Note that there are dozens or hundreds of white mice, dozens or hundreds of pigeons, rhesus monkeys...
Oh, rhesus monkeys are precious. All other experimental animals are dead. Relatively harmless drugs are screened out and then tested on rhesus monkeys.
Even when he was studying in his previous life, in order to let clinical medicine students experience it and conduct a 50% lethal dose experiment, a class would have to be divided into 8 groups, with 6 to 8 mice in each group. Do the math, no matter what, you need 48 to see if it's good or bad.
Unlike here, there are only two or three animals of the same species for each transmission route and each type of bat. The odds were so low that he was frightened. Whether it can produce results or not, I feel it is purely a matter of character.
In other words, could it be possible to successfully issue an early warning? Perhaps the national destiny of the Magic Council is at play?
Gretel answered without hesitation, and Pastor Matthew kept these unknown knowledge in his heart one by one. Even if God knows what it can be used for, if others are willing to share it generously, it is worth his or her efforts to help.
"Why do you have to have half male and half female? We have been looking for boars and rams for a long time..." Especially boars. Many of the young boars were killed and eaten, and the breeding pigs were not so easy to borrow. They were just a bag of tears. Finally, as a last resort, I went to catch a group of wild boars in the woods...
“Because of different diseases, the probability of illness in male and female individuals may not be the same…
Alas, poor... Gretel looked around the corral, correction, animal laboratory, and shed a handful of tears of sympathy for himself in his heart. According to the orthodox experimental design method, doubling the number of experimental animals may not be enough, but...
He could hardly afford to support him at this point.
Even if you can afford it, there are no manpower to take care of it. If there are people to take care of it, there are not that many manpower to take care of the animals one by one and measure their body temperature...
Alas, no wonder those who make new drugs are big companies. When his status rises in the future, he must ask the Magic Council for a research team to help him raise experimental animals...
Grete gave himself another reason to work hard to upgrade. Holding a notebook, Pastor Matthew measured the animal's temperature once, and he made a note next to it:
"Hare No. 12, body temperature is 39 degrees... Well, let's order two, 39.2 degrees. The food intake is normal, the spirit is normal, the defecation is normal, there are no abnormal secretions from the eyes, nose, and mouth, and the health is good... I told you this Why do we need to measure the rabbit if it’s normal?”
"Then you have to measure it! - Besides, how can you guarantee that it's definitely normal?!"
"I can communicate with it! Even the servants of the God of Nature can do it - right, you can't?!"
"Well……"
Grete, who is seriously partial to science, sneaks in, and Nordmark, except for healing, does not know any other skills of the God of Nature priest...
(There will be another update in five minutes)
Thanks to x-on:lof Xueyu, x-on:book friend 20210308190445235 for the reward of 100 starting coins
Please subscribe, ask for monthly tickets, please recommend votes...