Chapter 1285 Research Reopens. Blood-Replenishing Magic Is Too Difficult!
No matter how much the elves screamed and wailed, they could only hold on to the information given by Gretel and gnaw to death with difficulty and pain.
Half of the time is spent chewing information, and the other half of the time, I have to endure the torture of the laboratory:
"Aseptic operation! Aseptic operation! Don't pick up things that fall on the floor!"
Snapped! The transparent hemostatic forceps flew over and struck accurately on the radial styloid process of the offender, causing a whining sound.
"Who told you to put the lid down!"
Snapped!
The transparent hemostatic forceps appeared and disappeared, and the elf who made the mistake was at least a seventh- or eighth-level warrior, so he refused to escape.
"Don't touch the folded surface of the other glove with your gloved hand! Take off the gloves, go out and wash your hands, and put the gloves back on!"
Snapped!
The elves sighed, and so did Gretel. He really didn't want to train these elves if he didn't have to. God knows how difficult it is to have sterile operating standards ingrained into your bones and make every move and move without making any mistakes——
It is common for medical students who have just entered internship, even if they have been trained for several years, to be scolded or kicked out of the operating room;
It is also common for senior and mature doctors without special training to make mistakes when putting on and taking off protective clothing, face shields and masks, leading to self-infection;
As for ordinary people, the so-called wearing masks, sterilization, and sealing off areas can basically only reduce the probability of being infected. To say that you are 100% sure that you will not be tricked, basically, cough...
It can only be said that it is fate to be hit, and it is also fate not to be hit.
Of course, taking good protection in the face of the epidemic can reduce the chance of getting sick. Even if you are accidentally infected and inhale the smallest amount of virus, it will take a longer time to replicate and cause illness, and it will also give the body enough time to react to synthesize antibodies.
The more antibodies there are, the less damage the human body will suffer, the symptoms will be milder, and the person will feel better...
Just like Gretel now. The more assistants you train, the more assistants you have to help them do low-end tasks, such as collecting umbilical cord blood from rabbits. As for the sterile operation error, the removed umbilical cord blood cannot be used?
Anyway, there are dozens of rabbits to give birth in the first batch. A female rabbit gives birth to 7 to 8 rabbits in one litter. In total, there are hundreds of umbilical cord blood.
If you still can't practice it, there will be a second batch, a third batch, a fourth batch of rabbits... About every three days, a batch of rabbits will be produced, and the cycle will be monthly.
After practicing with so many rabbits, even the stupidest and most careless elf can reach the state of "without others, but with familiarity".
With so many assistants, Grete can focus his energy on more valuable things. For example, umbilical cord blood is directly injected into rabbits, and then targeted mutagenesis is attempted;
For example, throw spells at umbilical cord blood in vitro and try targeted mutagenesis;
For example, try to extract stem cells from umbilical cord blood;
For example, he tried to take unknown cells that had been isolated after multiple centrifugations, and brushed each cell with the spell configuration he designed to see if it could be induced to differentiate into blood cells in a targeted manner;
For example, for stem cells to differentiate into blood cells, they still need some environment, such as plasma. Grete had to find ways to create a working environment for them, and used his imagination to create seven or eight dozens of versions...
Buzz buzz, buzz buzz...
The centrifuge spins hard. Gretel had a thick record book in his hand, with a pen rolled on the root of the oak stick, and kept recording:
"In vitro experiment plan No. 1, add 2% hare serum to the culture medium... Targeted mutagenesis spell model No. 1... After 72 hours, absorb the suspension and make a smear for observation... A large number of bacteria appeared in the suspension, and the experiment failed..."
"Test tube No. 2...smear microscopy showed no viable cells...repeated three times to confirm that all cells were dead, the experiment failed..."
"Test tube number three..."
"Plan No. 2, 2% hare serum, 0.5% penicillin, 0.5% streptomycin... Directed mutagenesis spell model No. 1..."
"Plan No. 3, 2% hare serum, 0.5% penicillin, 0.5% streptomycin, releases anticoagulant magic... Targeted mutagenesis spell model No. 1..."
"Ahhhhh..."
Grete held his head and screamed. If you can’t achieve results, what’s the problem?
Is there a problem with the spell model, or is there a problem with the extraction of hematopoietic stem cells, or is the living environment of hematopoietic stem cells unsuitable?
Inadequacy is almost a given. Cells are very delicate things, and they are especially delicate when cultured in vitro.
Grete didn't need to study in depth. He thought about it with his superficial physiological and biochemical knowledge, and he knew that there needed to be enough oxygen in the culture medium, and of course, the proportion of carbon dioxide also needed to be appropriate;
Suppressing bacteria is necessary, but problems may arise if too much penicillin and streptomycin are added;
To generate red blood cells, additional iron is necessary, which requires transferrin - then the question arises, how to extract, isolate, or manufacture, human transferrin, or rabbit transferrin, add In the culture medium?
Oh, by the way, it seems that insulin needs to be added to supply enough sugar to the hematopoietic stem cells... So how should insulin be added?
It is difficult to do in vitro experiments, and it is also difficult to do in vivo experiments. Not only is it difficult to do, but you also have to slap the table with the elves while they look at them with surprise, fear, and contempt:
"Learn to draw blood! - Give you one day, everyone must learn to draw blood from rabbits!"
"Learn how to use a syringe correctly! Learn to identify blood vessels! Learn to draw blood from the rabbit's veins correctly and safely! Each rabbit should draw no less than 15 ml of blood and no more than 20 ml each time!"
“Learn about smears, microscopy, and counting blood cells!”
"Learn the magic of blood cell counting!"
The elves cried out. For those who serve nature, it is quite acceptable to collect umbilical cord blood from rabbits. After all, after the baby rabbit is born, the umbilical cord is useless anyway, and wasting umbilical cord blood is also a waste;
But, grab rabbits one by one, immobilize them, control them with magic, draw their blood...
Every whine, every struggle of the body in the hands, warm breath blowing on the arms, wet eyes looking at myself pleadingly...
The stronger the healing ability, the deeper the connection between the elves and nature, and the more sensitive they are to the emotions of animals; every bit of fear and pain of the experimental animals is like cutting directly into their hearts.
"No...can you please stop torturing them..."
"The purpose of conducting experiments on rabbits is to find a way to replenish blood for soldiers who have lost too much blood! The faster and more accurately you do it, the faster the research progresses, and the less the rabbits will suffer!"
Gretel suppressed their resistance without hesitation:
"Enhancing the recovery ability of warriors is to enhance the combat effectiveness of warriors! - If the warriors cannot withstand it, the plants and animals that will suffer, and the nature that will suffer, will be hundreds, hundreds, and thousands of times more than the dozens of animals in the laboratory!"
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